Figure 1
From: Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells
available at www.bloodspot.eu. (B) Cell proliferation curves of THP-1 cells transduced with a lentivirus encoding KLF4 or control cassette. Cells were cultured in the presence of 3 μM doxycycline (n = 3). (C) Representative microscopic images of THP-1 cells, as in (B). Cells were treated with 3 μM doxycycline for the indicated time periods, harvested, and cytospun onto glass slides. Diff–Quik staining (modified Giemsa staining) was performed on each of the slides (original magnification: ×20, scale bar 50 μm). Data are presented as mean ± SEM. ***P < 0.001, by two-tailed Student’s t test.
KLF4 induces monocytic differentiation of AML cells. (A) Box plot showing the expression levels of KLF4 in primary AML cells (AML t(15; 17), n = 54; AML inv(16)/t(16;16), n = 47; AML t(8;21), n = 60; AML t(11q23)/MLL, n = 43; AML complex, n = 48) or normal hematopoietic cells of various lineages (HSC hematopoietic stem and progenitor cells, n = 6; MPP multipotent progenitors, n = 2; CMP common myeloid progenitors, n = 3; GMP granulocyte-monocyte progenitors, n = 7; MEP megakaryocyte-erythrocyte progenitors, n = 4; early_PM early promyelocytes, n = 3; late_PM late promyelocytes, n = 3; MY myelocytes, n = 2; MM metamyelocytes, n = 3; BC band cells, n = 4; PMN polymorphonuclear cells, n = 3; Mono Monocytes, n = 4) (GSE42519 and GSE13159). Data were retrieved from the BloodSpot database30. The database is freely
