Figure 2

Selective upregulation of DPYSL2A upon exogenous expression of KLF4 in AML cells. (A) Identification of genes potentially regulated by KLF4. The top 1000 genes upregulated in Nras mut⁺ mouse HSPCs (GSE45194), Klf4-overexpressing mouse Tot2 cells (GSE38810), and KLF4 high-expressing AML patients (GSE22845) were extracted. A list of 26 genes that were commonly upregulated among these three datasets is shown. (B) The expression levels of 26 candidate genes from (A) in THP-1 cells with exogenous KLF4 expression. Cells were treated with 3 μM doxycycline for 24 h to induce KLF4 expression, and total RNA was extracted and analyzed by qRT-PCR. Values are normalized to those of control vector-transduced cells (n = 3). (C) The expression levels of DPYSL family members in THP-1 cells with exogenous KLF4 expression. Cells were treated with 3 μM doxycycline for 24 h to induce KLF4 expression, and total RNA was extracted and analyzed by qRT-PCR. Values are normalized to those of control vector-transduced cells (n = 3). (D) Immunoblot analysis of KLF4, DPYSL2A, and GAPDH in THP-1 cells transduced with a lentivirus encoding KLF4 or control cassette. Cells were treated with 3 μM doxycycline for 48 h and then lysed for protein extraction. (E) Box plot showing the expression levels of DPYSL2 in primary AML cells (AML t(15; 17), n = 54; AML inv(16)/t(16;16), n = 47; AML t(8;21), n = 60; AML t(11q23)/MLL, n = 43; AML complex, n = 48) or normal hematopoietic cells of various lineages (HSC hematopoietic stem and progenitor cells, n = 6; MPP multipotent progenitors, n = 2; CMP common myeloid progenitors, n = 3; GMP granulocyte-monocyte progenitors, n = 7; MEP megakaryocyte-erythrocyte progenitors, n = 4; early_PM early promyelocytes, n = 3; late_PM late promyelocytes, n = 3; MY myelocytes, n = 2; MM metamyelocytes, n = 3; BC, band cells, n = 4; PMN polymorphonuclear cells, n = 3; Mono monocytes, n = 4) (GSE42519 and GSE13159). Data were retrieved from the BloodSpot database³⁰. The database is freely