Figure 1 | Scientific Reports

Figure 1

From: Galectin-3 gene deletion results in defective adipose tissue maturation and impaired insulin sensitivity and glucose homeostasis

Figure 1

In vitro adipogenic differentiation of SVF cells. Lgals3 mRNA levels from SVF cells of WT mice cultured in normal control (Ctr) or adipogenic (Adip) medium (a); representative images of adipogenic differentiation of SVF cells isolated from SAT of WT and Lgals3−/− mice, cultured in control or adipogenic medium for 14 days (original magnification, 10X or 20X) (b); oil-Red-O staining of WT and Lgals3−/− SVF cells induced to differentiation (c); qRT-PCR analysis of genes involved in adipogenesis (Dlk1, Cebpb, and Pparg) (d), lipid metabolism (Srebf1, Acaca, Fasn, and Pnpl + a2) (e), adipokine synthesis (Adipoq) (f), inflammation (Il6 and Ccl2) (g), and proliferation (Ccnd1) (h); and analysis of BrdU incorporation (i) from SVF cells induced to differentiation of WT and Lgals3−/− mice. Values represent the mean ± SD of two independent experiments (n = 3 mice per group per experiment). †P < 0.01 versus control medium; *P < 0.05, **P < 0.01, or ***P < 0.001 versus WT.

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