Figure 5 | Scientific Reports

Figure 5

From: Identification of the HECT E3 ligase UBR5 as a regulator of MYC degradation using a CRISPR/Cas9 screen

Figure 5

Targeting UBR5 in cell lines with MYC amplification. (A) Cell viability analysis using cell titer glow assay in cell lines upon knockout of UBR5. Data are normalized to cells transfected with sgNT. (B) Western blot analysis showing knockout of UBR5 and stabilization of MYC in cell lines with or without MYC amplification. (C) Localization of UBR5 (8q22.3) and FBXO32 (8q24.13) in proximity to MYC (8q24.21) on Chromosome 8, unlike FBXW7 (4q31.3, not shown). (D) Correlation of cell line copy number (CN) estimates of UBR5, FBXO32, and FBXW7 show co-amplification of UBR5 and FBXO32 with MYC amplifications. (E) Transcript-level distributions (shown as kernel density estimates of log2 FPKM values) of UBR5, FBXO32, and FBXW7 in cell lines as a function of MYC copy number demonstrate distinct expression upregulation of UBR5. (F) Transcriptional correlation of MYC and UBR5 level across CCLE. (G) Transcriptional correlation of MYC and UBR5 level across TCGA. (H) Transcript-level distributions of MYC as a function of MYC copy number.

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