Figure 2

Sequencing metrics. Panels a-d show the effect of read depth on (a) sequencing saturation, (b) total genes detected, (c) median genes per cell and (d) median unique molecular identifier (UMI) counts per cell. Panels e and f show cell numbers pre and post quality control for (e) unstimulated and (f) stimulated samples. Unstimulated datasets are shown in blue and stimulated datasets in red.