Table 4 Neddylation pathway and substrate proteins common in the mass spectrometry of immunoprecipitates of rub1∆[HA-rub1] (all three experiments) and rub1∆[HA-PfN8] (at least once).
From: Characterization of Plasmodium falciparum NEDD8 and identification of cullins as its substrates
ID | Protein | rub1Δ [HA-PfN8] | rub1Δ [HA-rub1] | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment 1 | Experiment 1 | Experiment 2 | Experiment 3 | ||||||||||
Sc | Co | UP | Sc | Co | UP | Sc | Co | UP | Sc | Co | UP | ||
P52286 (SKP1_YEAST) | Suppressor of kinetochore protein 1 | 18.1 | 32.5 | 6 | 17.2 | 23.7 | 5 | 3.9 | 26.3 | 3 | 38.3 | 28.9 | 6 |
Q99344 (UBA3_YEAST) | NEDD8-activating enzyme E1 catalytic subunit | 4.2 | 3.7 | 1 | 17.0 | 14.0 | 4 | 0.0 | 5.7 | 2 | 8.7 | 5.0 | 1 |
Q08273 (RBX1_YEAST) | RING-box protein HRT1 | 18.7 | 31.4 | 2 | 15.0 | 11.6 | 1 | 28.9 | 24.0 | 3 | 7.3 | 11.6 | 1 |
P24814 (GRR1_YEAST) | SCF E3 ubiquitin ligase complex F-box protein GRR1 | 61.8 | 18.2 | 20 | 48.8 | 9.9 | 9 | 15.2 | 7.7 | 8 | 2.1 | 0.7 | 1 |
Q12059 (ULA1_YEAST) | NEDD8-activating enzyme E1 regulatory subunit | 2.4 | 2.6 | 1 | 12.3 | 8.2 | 4 | 16.0 | 14.1 | 7 | 12.2 | 12.8 | 5 |
P47005 (DAS1_YEAST) | F-box protein DAS1 | 71.7 | 31.7 | 19 | 15.8 | 6.8 | 4 | 0.0 | 6.3 | 3 | 25.3 | 12.5 | 9 |
P38352 (SAF1_YEAST) | SCF-associated factor 1 | 48.0 | 26.7 | 13 | 6.8 | 5.0 | 3 | 0.0 | 11.8 | 5 | 127.7 | 43.3 | 19 |
