Figure 3
From: PRMT1 inhibition induces differentiation of colon cancer cells
PRMT1 inhibition/knock down mediates differentiation phenotype in colon cancer cells. (a) Effect of PRMT type 1 inhibitor (MS023) and its inactive analog (MS094) on colon cancer cell (HT-29) growth and ALP activity, 5 days after treatment. The data is normalized relatively to DMSO treated cells. Graphs represent mean ± SEM from three independent experiments. Graph was made using GraphPad Prism version 8.4.3 for Windows, GraphPad Software, San Diego California USA (www.graphpad.com). Statistical significances of ALP activity and cell viability between MS023 and MS094 treated cells are indicated. Chemical structures of the compounds are shown on the right side of the graph. (b) Effect of PRMT1 knock down on HT-29 growth and ALP activity, 5 days after treatment. Groups: non treated cells, DharmaFECT – cells treated with transfection reagent alone, siCon, cell transfected with relevant control siRNA, siPRMT1—cells transfected with siPRMT1. Graphs represent mean ± SEM from three independent experiments. Statistical significance of ALP activity and cell viability between siPRMT1 and others groups is indicated. (c) Western blot analysis of PRMT1 expression in the indicated groups of HT-29 cells, 5 days after transfection. Full-length blots are presented in Supplementary Fig. S2.
