Figure 4
From: PRMT1 inhibition induces differentiation of colon cancer cells
MS023 stabilizes PRMT1. (a) Cellular thermal shift assay of MS023 in HT-29 cells. Graph represents relative PRMT1 band intensities of the indicated samples ± SEM from three independent experiments. Representative PRMT1 Western blots of each sample are shown. Statistical significances between MS023 and DMSO are indicated. The analysis was performed simultaneously in the MS023 and DMSO samples with same time of the exposure. Full-length blots are presented in Supplementary Fig. S3. (b) Isothermal (at 63 °C) dose response fingerprints in HT-29 cells. Graph represents relative PRMT1 band intensities of the indicated samples ± SEM from three independent experiments. Graphs in a and b were made using GraphPad Prism version 8.4.3 for Windows, GraphPad Software, San Diego California USA (www.graphpad.com). Representative PRMT1 and actin Western blots of each sample are shown. Full-length blots are presented in Supplementary Fig. S4.
