Figure 6
From: Factors affecting stability of plasma brain-derived neurotrophic factor
BDNF increases following freeze–thaw cycles. (A) The quantity of BDNF in the plasma collected in K2EDTA tubes from a prior study increased in women, over repeated freeze-thaws. The values obtained from the plasma of each woman after each freeze–thaw cycle are connected by the lines in the plot. (B) The mean quantity of BDNF significantly increased as the number of repeated freeze–thaw cycles increased (p ≤ 0.0001, one-way ANOVA with Tukey’s post hoc). BDNF: brain-derived neurotrophic factor. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001. Data in (A) are presented as individual plasma samples, while data in (B) are presented as a box plot (25th, 50th (median), 75th percentiles). Dotted lines indicate the expected range of the means for the K2EDTA plasma separator (calculated to be ± 47.9%, based on inter-assay CV and the mean concentration of BDNF recovered from each plasma separator at Time 0). + : indicates the mean.
