Figure 2
Cancer cell cytotoxicity of TRAIL-ATNCs via TRAIL receptors. (A) Cell viability following treatment with TRAIL, TRAIL-ATNC, or TRAIL-ATNCIL4rP. MDA-MB-231 cells were incubated with proteins for 24 h and then measured by EZ-count assay. Data represent means ± SEM. (B) The apoptosis inducing ability of TRAIL or TRAIL-ATNC was analyzed with Annexin V and propidium iodide (PI) double staining using flow cytometry. The percentage of annexin V/PI double-negative (viable) cells was plotted. (C) Apoptosis inducing ability of TRAIL or TRAIL-ATNC was blocked by anti-DR4/DR5 antibodies. MDA-MB-231 cells were pre-incubated with DR4/DR5 blocking antibody or control IgG for 1 h and were incubated with TRAIL or TRAIL-ATNC for 3 h. Cell viability was measured with Annexin V and PI double staining using flow cytometry.
