Figure 3 | Scientific Reports

Figure 3

From: Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction

Figure 3

Enzyme reactions with Lgd proteins using 3-keto-LG as the substrate. The reaction mixture containing 50 mM HEPES-NaOH (pH 7.5), 0.3% 3-keto-LG, 1 mM β-NAD+, 1 mM MnCl2·4H2O, and appropriate amount of the recombinant protein(s) were incubated at 30 °C for 30 min. β-NADH was added to the mixture containing LgdC as one of the enzymes. Ten microliters of the reaction mixture were used for TLC analysis. B1, B2, and C in the figure represent LgdB1, LgdB2, and LgdC, respectively. The dark green and the violet spots indicated by the arrow a and b correspond to the reaction product of LgdB1 and LgdB2, respectively.

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