Figure 4 | Scientific Reports

Figure 4

From: Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction

Figure 4

LgdC reaction using glucose as the substrate (reverse reaction). The reaction mixture for lane 1: 50 mM Tris-HCl (pH 9.0), 0.1% glucose, and 1 mM β-NAD+; that for lane 2: 10 μg/ml LgdC was added to the reaction mixture for lane 1; that for lane 3: 50 mM HEPES-NaOH (pH 7.5), 0.1% LG, 1 mM β-NAD+, 1 mM MnCl2·4H2O, and 5 mg/ml each of LgdA, LgdB1, and LgdB2. The reaction was carried out at 30 ℃ for 15 h and 10 μl of the reaction mixture was used for TLC analysis. An arrow indicates the spots corresponding to the product of the reverse reaction of LgdC and that of the forward reaction by LgdA, LgdB1, and LgdB2.

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