Figure 1 | Scientific Reports

Figure 1

From: Disease-related Huntingtin seeding activities in cerebrospinal fluids of Huntington’s disease patients

Figure 1The alternative text for this image may have been generated using AI.

N17 mutations in recipient cell lines altered sensitivity of seeding-induced mHTT aggregation. (a) A diagram illustrates the constructs of GFP-tagged mHTT-exon 1 (46Q) fragments, which are used to generate stable cell lines. (b) A diagram illustrates the generation of mHTT aggregates (fibrils) and seeds from monomeric recombinant mHTT-51Q proteins. (c) Cell lines generated by stably transfected HEK-293 cells with GFP tagged mHTT-exon 1 (46Q) variants were used as recipient cells of the seeding assay. Constructs of the transgenes are labeled on the left of the panel. Recombinant mHTT-exon 1 (51Q) in the forms of monomers, fibrils and seeds were added directly to the cultured cells for 3 days. Representative confocal images of the cells are shown. Bright green punctae are intracellular mHTT-GFP aggregates induced by seeding. N/T = non-treated. (d,e) The number of HTT-GFP aggregates was quantified using ImageJ and presented as mean ± s.e.m., n = 5–10, **p < 0.01.

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