Figure 2 | Scientific Reports

Figure 2

From: Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome

Figure 2

Assessment of mucosal eosinophil activation. (A) Quantitative gene expression analysis of the secretory activity-related synaptosomal-associated protein 23 (SNAP23). Individual values represent the fold change with respect to the average of the HC group (HC, n = 10; IBS-D, n = 16). (B) Representative images of mucosal eosinophils. White arrow indicates loss of granular content of cytoplasmic granule. The boxed areas in (B) are shown in (Bi-Bii) at higher magnification. Black arrowhead in the insert Bi (in IBS-D) shows a partially degranulated granule with vesico-tubular structures and sombrero-like vesicles indicative of active degranulation. Black arrow in Bi and Bii indicates granule enlargement, also indicative of PMD process Magnification: bars indicate 2 µm. (C) Quantification of degree of PMD. Percentage of eosinophils with moderate, low or no degranulation (HC, n = 13; IBS-D, n = 11). (D) Percentage of granular cytoplasmic area and the degranulated area of granules in eosinophils in each group, and number of lipid bodies and granules per eosinophil. Individual values represent an average of counts in different eosinophils in different sections of each sample *P < 0.05; ***P < 0.001. HC, healthy control; IBS-D, diarrhea-predominant IBS (HC, n = 11–12; IBS-D, n = 11).

Back to article page