Table 1 Small angle X-ray scattering data collection and experimentally derived parameters of MtbGmhA. Rg = radius of gyration. Dmax = maximum particle dimension.
MtbGmhA | |
|---|---|
Data collection parameters | |
Instrument | SAX Space (Anton Paar) |
Beam geometry | 10 mm slit |
Wavelength (Å) | 1.5418 |
Q range (nm−1) | 0.10–3.00 |
Exposure time (min) | 30 |
Concentration (mg/ml) | 1.22 |
Temperature (K) | 283 |
Structural parameters | |
I(0) (cm−1) [from P(R)] | 25,650.00 |
Rg (nm) [from P(R)] | 2.73 ± 0.11 |
I(0) (cm−1 from Guinier) | 26,737.10 ± 492.71 |
Rg (nm) (from Guinier) | 2.48 ± 0.17 |
Dmax (nm) | 7.9 |
Porod volume estimate (nm3) | 130.29 |
Molecular-mass determination | |
Molecular mass Mr (kDa) I(0) | 93.2 |
Calculated monomeric Mr from sequence (KDa) | 24.105 |
Software employed | |
Primary data reduction | SAXSquant |
Data processing | PRIMUSQT |
Ab initio analysis | DAMMIF |
Validation and averaging | DAMAVER |
Computation of model intensities | CRYOSOL |
3D graphics representations | PyMOL |
