Table 2 Oligonucleotide primers used to amplify and sequence mitochondrial (mtDNA) and nuclear DNA sequences (nuDNA).
From: Genetic origins and diversity of bushpigs from Madagascar (Potamochoerus larvatus, family Suidae)
Genomic region | Locus | Primer name | Primer sequence (5′ → 3′) | Target size (bp) | Source |
|---|---|---|---|---|---|
mtDNA | CR | CR L CR H | CCAAGACTCAAGGAAGGAGA GGCGCGGATACTTGCATGTG | 1036 | |
cytb | LT4724 H15915R | CGAAGCTTGATATGAAAAACCATGGTTG GGAATTCATCTCTCCGGTTTACAAGAC | 1140 | ||
nuDNA | GPIP | GPIP1 GPIP6 | TGCAGTTGAGAAGGACTTTACTT GAAGTTACAGGGCATCATCTTG | 507 | |
MC1R | MC1R-F MC1R-R | AGTGCCTGGAGGTGTCCATTCAC CGTAGATGAGGGGGTCCAGGATAGA | 795 | ||
SLA-6 (class I) | SLA-6F SLA-6R | TCAGCCYCTCCCTGTTMTAG GTTCCTGCACCCCCTTASAC | 314 | Lee et al., unpublished | |
SLA-DQB1 (class II) | DQB1-e2_F DQB1-e2_R | GCCTGACTGACGCGGTATCTC GAGTGCCTGCCCGCC | 315 | Lee et al., unpublished | |
BAG6 (class III) | BAG6-F BAG6-R | CCCTTGCTCCCTCTTCTACC GCTTCTCATGCAGCCTGTG | 895 | This study |
