Figure 3
Comparative transcriptomic analysis of PVEC vs. ABEC following co-culture with NPC indicate major changes in PVEC not ABEC. (a) Principal component analysis (PCA) of transcriptome. Colors of circles refer to the origin of samples: green PVEC and purple ABEC, light versus dark shades indicate mono vs. NPC co-culture conditions. (b) Venn Diagram of DEG in PVEC vs. ABEC (grey ellipse), PVEC + NPC vs. ABEC + NPC (orange ellipse), ABEC vs. ABEC + NPC (pink ellipse) and PVEC vs. PVEC + NPC (blue ellipse). (c) Eight different self-organizing maps (SOM) patterns of gene expression profiles were identified amongst the 321 genes that were different in PVEC vs. PVEC + NPC and also in PVEC vs. ABEC. The upper panels display patterns whose gene expression is upregulated in PVEC + NPC as compared to baseline PVEC. Data is shown as standard boxplots (box and whisker plots), depicting the median bar and the dots representing the outliers. Lower panels depict patterns whose gene expression is downregulated in PVEC + NPC as compared to baseline PVEC. Treemap representation of GO_bp (biological process) using REVIGO (reduced visualization of GO) corresponding to (d) Pattern I, (e) Pattern VI and (f) Pattern VIII.
