Figure 2

Immunoblotting analysis of AdeT1 expression in E. coli KAM32 from three plasmids. The pAdeT1 plasmid was constructed as described before³⁵, while pAdeT1-His6 contains a 6 × Histidine tag on the C terminus of the protein encoding gene. The pAdeT1*-His6 plasmid is a similar construct, with the adeT1 gene in frame with a start codon and with a 6 × Histidine tag on the C terminus. Cells were induced with 0.5 mM IPTG at OD₆₀₀ ~ 0.6. After 2 h at 37 °C, samples were taken for analysis. A full-length version of the immunoblot is provided in Supplementary Fig. S3.