Figure 8

Antibiotic susceptibility of E. coli KAM32 carrying either pUC18 or pAdeT1*-His6. Data of two biological replicates (i.e. independent transformations), (1) and (2), are shown. E. coli KAM32 carrying either pUC18 or pAdeT1*-His6 were cultured to OD₆₀₀ 0.6 before induction with 0.5 mM IPTG. After 2 h, a defined amount of cells were dropped on agar plates containing 0.5 mM IPTG, 100 µg/mL ampicillin and varying concentrations of chloramphenicol (top), tetracycline (middle) or erythromycin (bottom). Plates were incubated at 37 °C for 18 h before colonies of E. coli KAM32 carrying pAdeT1*-His6 were analysed by immunoblotting to confirm protein expression. Full-length blots are shown in Supplementary Figs. S12 and S13.