Figure 5

Initial increased tumorigenic activity in low-autophagy compartments is oncogene-independent. (A) GSEA of transcripts measured in MCF10A-BMPR1B⁺ compared to MCF10A cells. Gene sets shown are Positive regulation of Autophagy, Autophagy, Regulation of Autophagy. (B) Distribution of R⁺ and G⁺ cells in FACS profiles of RFP-GFP-LC3B–transduced MCF10A-BMPR1B⁺ cells. (C) Representatives pictures of soft agar colonies from R⁺ or G⁺ MCF10A-BMPR1B⁺ cells after three more weeks of culture. The scale bar represents 200 μm. Dot plot showing the frequency of soft agar clonogenic activity comparing FACS-selected R⁺ or G⁺ MCF10A-BMPR1B⁺ cells.