Table 1 MIC of D-cycloserine against different strains of E. coli and B. subtilis expressing ddl.
Bacterial strain | Plasmid vector | Inserts and their size | MIC of D-cycloserine (µg/mL) | MIC of vancomycin (µg/mL) |
|---|---|---|---|---|
E. coli ɑ-select | ||||
EC121 | pGEM-TEasy | Empty (vector control) | 8 | ND |
EC126 | pGEM-TEasy | intI-attI-ddl6 (2024 bp) | 16 | ND |
EC127 | pGEM-TEasy | intI-attI-ddl7 (2024 bp) | 64 | ND |
EC206 | pGEM-TEasy | intI-attI-ddl6 c.490 C > T (2024 bp) | 16 | ND |
EC207 | pGEM-TEasy | intI-attI-ddl6 c.777G > T (2024 bp) | 64 | ND |
B. subtilis 168 | ||||
BS700 | pHCMC05 | Empty (vector control) | 8 | < 0.125 |
BS706 | pHCMC05 | pHCMC05:: ddl6 | 64 | < 0.125 |
BS707 | pHCMC05 | pHCMC05:: ddl7 | 64 | < 0.125 |
