Figure 3
From: α-Synuclein promotes IAPP fibril formation in vitro and β-cell amyloid formation in vivo in mice
adapted from Fig. 2a but presented with a different scale on the x-axis to allow direct comparison with ThT emissions curves in (b,c). Also, please note that the ThT emission exceeds the setting of the plate reader when co-incubating 2 µM hIAPP monomers with 35 and 70 µM hαSyn monomers (blue curves in b,c), hence the maximal ThT emissions for these reactions are likely larger than that displayed in (b,c). Data in (a–c) are presented as mean value + /− SEM. (d–g) TEM negative stain images of the resulting fibrils formed from 2 µM hIAPP alone (d), 2 µM hIAPP with 7 µM (e), 35 µM (f), and 70 µM (g) hαSyn, respectively. Scale bar is 400 nm in (d–g).
αSyn monomers dose-dependently cross-seed IAPP fibril formation. (a–c) Fibril formation ThT curves for 2 µM hIAPP monomers alone (black) and with 7, 35, and 70 µM hαSyn monomers (blue), shown are also curves for 7, 35, and 70 µM hαSyn monomers alone (red). Please note that (a) is
