Figure 4
From: α-Synuclein promotes IAPP fibril formation in vitro and β-cell amyloid formation in vivo in mice
Tail vein injection of αSyn enhances amyloid formation in hIAPPtg mice. Islet amyloid quantification in pancreas from tail vein injected hIAPPtg mice. (a) Representative images of Thio-S+ amyloid deposits in islets of wild type (wt; upper panel) and hIAPPtg (lower panel) mice tail vein injected with PBS (= control), and 7 µM of hαSyn monomers, fibrils or seeds, respectively. (b) Quantification of percentage Thio-S+ amyloid area/islets area of wild type and hIAPPtg mice tail vein injected with PBS (= ctrl.), 7 and 70 µM hαSyn monomers, fibrils or seeds, respectively. Please see “Amyloid analyses” in the Materials and methods section for information on numbers of islets and mice analysed for each category. Scale bar is 100 µm. Data are presented as mean + /− SEM, **P < 0.01, ***P < 0.001 (Student’s t-test).
