Figure 6 | Scientific Reports

Figure 6

From: Multiple myeloma hinders erythropoiesis and causes anaemia owing to high levels of CCL3 in the bone marrow microenvironment

Figure 6

Bone marrow plasma inhibited erythroid colony formation of HSPCs by increasing CCL3 levels in the MM microenvironment. (A) A CFC assay was utilized to quantify the BFU-E colony formation of CD34+ cells after co-culture with or without BM plasma from MM patients for 14 days. The absolute numbers of BFU-E colonies are shown on the right. (B) The concentrations of CCL3 in the bone marrow plasma cells and PB serum of 5TGM1 MM mice and control mice were detected with ELISA. (C) The concentration of CCL3 in the bone marrow plasma cells of MM patients was determined with ELISA (normal, n = 40; NDMM pts, n = 32). (D) Kaplan–Meier analysis showed shorter overall survival in MM patients with high levels of CCL3 based on the GEO dataset GSE2658. (E) The clinical data indicated that the more severe the bone disease is (MBD 3–4), the greater the decrease in haemoglobin in MM patients (MBD: 0–2, n = 47; MBD: 3–4, n = 102).

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