Table 4 Oligonucleotide primers used to determine chlamydial prevalence and C. psittaci genotype.
From: Species, sex and geographic variation in chlamydial prevalence in abundant wild Australian parrots
Primer | Specificity | Target | Sequence (5′–3′) | Size (bp) | References |
|---|---|---|---|---|---|
16SIG | All Chlamydiales | 16S rRNA | F: CGGCGTGGATGAGGCAT R: TCAGTCCCAGTGTTGGC | 298 | Everett et al.72 |
F3/B3 | C. psittaci | Cpsit_0607 | F: AGAACCGGATTAGGAGTCTT R: GCTGCTAAAGCGAGTATTGA | 263 | Jelocnik et al.73 |
gidA | C. gallinacea | gidA | F: TTTATCATTAAAACAGCGTGGTTTC R: GAGGTGGCGATCTTTTTCAGAG | 331 | Li et al.74 |
CTU/CTL | C. psittaci (and C. gallinacea)41 | ompA | F: ATGAAAAAACTCTTGAAATCGG R: CCAGCTTTTCTAGACTTCATCTTGTT | 1070 | Denamur et al.77 |
