Figure 4
From: Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
Three-dimensional AGO protein structure predictions. (a) Crystal structure of the human AGO2-miR-20a complex. N-terminal = green, L1 = red, PAZ = cyan, L2 = magenta, MID = yellow, PIWI = salmon. (b) AtAGO1 (steel blue) and Hs-miR20 (orange, PDB ID 4F3T:R). Residues of the D-E-D-H motif are in purple. The nucleotide specificity loop (NSL) in pink. (c) StAGO15 (green) and Hs-miR20 (orange). Residues of the G-E-Q-R motif in light cyan. The NSL in yellow. (d) Figure b and c merged. (e) Close view of the NSL in figure d (upper square). Nitrogen (red) and oxygen atoms (blue). The replacement of Asparagine (ASN, 687) in AtAGO1 to Phenylalanine (PHE, 583) in the StAGO15 NSL is highlighted with a white star (PHE, 583) and a white ring (ASN, 687). (f) Close view of the D-E-D-H/G-E-Q-R motives in figure d (lower square, Hs-miR20 removed). Nitrogen (red) and oxygen atoms (blue). StAGO15 residue labels are placed above the AtAGO1 labels.
