Figure 3
From: Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals
The bioactive factor(s) in EqMDEC CM are small in size and temperature-stable. MTT assays of MDA-MB-231 cultured for 48 h in the presence of EqMDEC CM that was manipulated, as described. DMEM controls were also included for each experiment. (A) EqMDEC CM was fractionated with centrifugation filters. (B) EqMDEC CM was either boiled at 100 °C for 10 min, frozen at − 80 °C for one week, or lyophilized and reconstituted to its original volume. (C) EqMDEC CM was pre-treated with proteinase K. Significant differences are either depicted by asterisks: ****p < 0.0001, or by different letters. ns: not significant, n = 3. Data are presented as the mean ± standard deviation.
