Figure 4
From: Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals
Cryopreservation of EqMDEC affects the bioactivity of the secretome. (A) MTT assays of MDA-MB-231 cells cultured for 48 h in the presence of CM that was collected either from freshly isolated EqMDEC (non-CP) or previously cryopreserved EqMDEC (CP). (B) Non-CP and CP Eq MDEC look similar morphologically (i), have similar population doubling times (PDT) (ii), and display similar mammosphere-forming capacities (iii). (C) Non-CP and CP Eq MDEC differ in the percentage of CD44/CD29 double-positive cells detected by flow cytometry (i) while the percent of CD44/CD49f. double-positive cells is similar regardless of cryopreservation status (ii). Significant differences are depicted by different letters, n = 3. Data are presented as the mean ± standard deviation.
