Figure 4
From: Novel multimodal molecular imaging of Vitamin H (Biotin) transporter activity in the murine placenta
Biotin–Fluorescein injection reveals biotin transport from the mother to the fetus. Fluorescent images of placental and fetal histological sections, harvested from B6 E14.5 pregnant females 60 min post-injection of biotin–Fluorescein via tail vein (a–d). (b) Biotin–Fluorescein was accumulated in the placenta within RBCs in the MBS and fetal RBCs in the EBS of the labyrinth. Higher magnification images are shown for (c) MBS and (d) EBS. (e–h) biotin–Fluorescein was transported to the Fetus and accumulated within: RBCs in fetal thymus, heart and liver. Higher magnification images of biotin accumulation within (f) the fetal thymus and (g) the fetal Left atrium and left ventricle. (h) Representative high magnification (50 µm) image of biotin accumulation within the fetal RBCs. Scale bars = 1000 μm (a,e), 200 μm (b,f,g) and 50 μm (c,d,h). MBS maternal blood spaces, EBS embryonic blood spaces, Lab Labyrinth, TGC trophoblast giant cells, Sp Spongiothrophoblast cells, LV left vertical, LA left atrium, Thy thymus. Blue, DAPI. Yellow, biotin–fluorescein.
