Figure 5
From: Novel multimodal molecular imaging of Vitamin H (Biotin) transporter activity in the murine placenta
Fluorescence microscopy of placenta from (left) pre-contrast biotin competition experiment, and (right) contrast-only experiments. Histological sections were harvested from a E14.5 pregnant mouse during SI reduction phase [15–30 min]. (a–c) Representative histological images following the administration of b-BSA-GdDTPA [10 mg, n = 6] for the original experiment, and (d–f) for biotin-competition experiment involving administration of native biotin (0.2 mg) three min prior to administration of b-BSA-GdDTPA [10 mg, n = 4]. (b,e) Biotin-only labeling exhibited aggregation of biotin in both versions of the experiment with smaller aggregates occurring for native biotin. (c,f) Co-labeling for biotin and albumin. The lack of co-localization in (f) suggests that aggregates in this panel were formed by native biotin, which preceded to “occupy” the biotin transporters in the placenta. [Green, biotin labeling with Avidin-FITC; Red, Avidin staining for albumin (b-BSA-GdDTPA)].
