Figure 1
From: Herpes simplex virus 1 targets IRF7 via ICP0 to limit type I IFN induction
Full-length ICP0 negatively regulates IFN-β promoter activity in response to multiple drivers. HEK-293T cells were transiently transfected with 50 ng of the IFN-β p125 promoter, 5 ng of TK renilla, and increasing concentrations (10, 50 or 100 ng) of full-length ICP0 as indicated. In addition, cells were cotransfected with RIG-I (A) or TBK-1 (B) or empty vector (EV) control, as indicated, and assayed for reporter gene activity 18 h posttransfection. Results are expressed as the mean ± SD in each case are representative of three independent experiments expressed as fold stimulation over unstimulated empty vector (EV) control. *p < 0.05, **p < 0.01 as determined by Student t test.
