Figure 1
Cutibacteria inhibited the capacity of S. epidermidis to form a biofilm. (a) Culture supernatant (CS) of C. acnes ATCC29399 inhibited biofilm formation by S. epidermidis s 1457 as seen by crystal violet staining. C. acnes was cultured in reinforced clostridial media (RCM) and fresh RCM was used as control. C. acnes CS or RCM was added to a final concentration of 25% (v/v) during growth of S. epidermidis for 6 or 24 h. (b,c) Dose-dependent inhibition of biofilm formation but not cell growth by C. acnes CS when applied to S. epidermidis 1457 (b) or S. epidermidis clinical isolate (c). (d) CS of several species of Cutibacteria inhibited S. epidermidis 1457 biofilm formation. (e) CS of several strains of C. acnes inhibited S. epidermidis 1457 biofilm formation. Data were expressed as mean ± SEM of a single experiment (n = 6) that was representative of 3 independent experiments. Differences were analyzed using the unpaired Student’s t test (b,c) or one-way ANOVA with Dunnett's test (d,e). Significance was shown as *P < 0.05, **P < 0.01, *** P < 0.001.
