Table 1 pH analysis of C. acnes CS (A), and chemical properties of biofilm inhibiting activity from C. acnes (B).
(A) | ||||||||
|---|---|---|---|---|---|---|---|---|
% C. acnes CS | 100 | 50 | 25 | 12.5 | 6.3 | 3.1 | 1.6 | 0 |
pH | 4.85 | 5.3 | 6.0 | 6.7 | 6.95 | 7.1 | 7.15 | 7.2 |
% RCM | 100 | 50 | 25 | 12.5 | 12.5 | 3.1 | 1.6 | 0 |
pH | 5.5 | 6.55 | 6.85 | 7.0 | 7.1 | 7.15 | 7.2 | 7.2 |
(B) | ||||||||
|---|---|---|---|---|---|---|---|---|
Manipulation | Biofilm inhibition | Suggested chemical property | ||||||
Ammonium sulfate precipitate | Not in precipitate | Not protein | ||||||
Heating at 100 °C for 10 min | Remains active | Heat resistant | ||||||
Proteinase digestion | Remains active | Not protein | ||||||
Lysozyme digestion | Remains active | Not glycoprotein | ||||||
Lyophilization | Activity lost | Volatile | ||||||
MW = 500 dialysis | Activity lost | MW < 500 | ||||||
