Figure 1 | Scientific Reports

Figure 1

From: Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer

Figure 1

PP VII mediates macrophage transformation associated with STING. (a–h) PP VII, a small molecular compound (a). CCK8 assays show the cell viability of RAW 264.7s (b) and THP-1s (c) incubated with PP VII at indicated concentration for 24, 48, 72 h separately. Data are depicted as mean + SD of triple samples from one representative experiment of two. The effect of PP VII on cell death for 24 h qualified by apoptosis in RAW 264.7s and THP-1s (d). Data are mean + SD of triplicate samples. ns no significant. Immunoblots analysis of RAW 264.7s and THP-1s stimulated by indicated cytokines for 24 h following a 3 h of PP VII pretreatment with indicated antibodies (e,f,h). RAW 264.7s left unstimulated only treated by PP VII for 24 h (g). Representative immunoblots form 2–3 independent experiments are depicted. If indicated, cells were stimulated with 100 ng/ml IFN-γ or 50 ng/ml IL-6 for 24 h. THP-1 cells were differentiated with PMA before any treatment and, unless stated otherwise.

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