Figure 2
From: Effects of cochlear hair cell ablation on spatial learning/memory
Vestibular, retinal, and central exclusion. Error bars represent standard deviation, *P < 0.05. All scale bars represent 20 μm. (A) Vestibular thresholds were determined to be the lowest intensity at which a characteristic Peak 1 could be identified in both positive and negative directions. Tracings represent the average of 256 responses. (B) Vestibular thresholds at 90 dpi shows no detectable physiologic vestibular difference between WT and DTR animals (0 dpi = P30, n = 10 mice per group). (C) Utricle cell counts per 130 μm2 section show a statistically significant reduction in DTR animals that were not physiologically significant. Each point represents the average of a striolar and extrastriolar count per animal (n = 10 mice per group). (D) H&E stained retina shows characteristic architecture: extraocular muscles (EOM), choroid coat (CC), outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL). (E) GCL cell counts were performed in an automated fashion using spot detection in the DAPI channel along 210 μm sections (40 μm original slice thickness). (F) No loss of RCG cells were observed at 2 and 10 dpi. Each point represents the average of 6 sections per eye (n = 8 eyes from 4 animals per group). (G) Striatal cells were counted in 420 μm2 sections (40 μm original slice thickness) using anti-NeuN with DAPI counter-stain. (H) Striatal nuclei (all cell types) counts were performed in an automated fashion using spot detection in the DAPI channel. (I) Striatal neuron counts were performed in an automated fashion using spot detection in the NeuN channel. (J) No loss of striatal nuclei or neurons were detected were observed at 2 and 10 dpi. Each point represents the average of 8 sections per hemisphere (n = 8 hemisphere from 4 animals per group).
