Figure 1
IL-28B alleviated papain-induced airway injury in mice. (A) Work flow for IL-28B treatment in papain-induced asthmatic mice. Briefly, mice were injected (h.p.) with plive-IL-28B or plive-vector or PBS on day 0 and were subsequently challenged with papain for 6 consecutive days. All mice were assayed on day 7. (B) Histologic sections of lung from PBS-, plive-vector- or plive-IL-28B- treated asthmatic mice were stained with Periodic Acid-Schiff (PAS) and analyzed by light microscopy. Scale bar = 100 μm. (C) Histological scores for assessment of lung injury were shown. (D) PAS staining for mucus production in lung epithelium from each group were shown. Scale bar = 50 μm. (E) Airway resistance was measured in response to increasing doses of aerosolized methacholine in mice (two-way ANOVA comparing plive-vector-treated and plive-IL-28B-treated mice). Data are representative of three or more independent experiments with 4 mice per group. Data are the mean ± SEM. *P < 0.05, ****P < 0.0001, ns = not significant. (Two-way ANOVA for E and student’s t-test for C). h.p., hydrodynamic injection; i.n., intranasally injection.
