Figure 5
IL-28B down-regulated IL-25 and TSLP expression in epithelial cells by targeting Erk. (A) Schematic diagram for in vitro culture: MLE-12 cells were treated with 200 ng/ml papain. After 4 h, IL-28B (100 ng/ml) was added and cocultured for 24 h. (B) The mRNA levels of TSLP and IL-25 in MLE-12 cells were determined by Real-time PCR. (C) Schematic diagram for ex vivo culture: Mice were treated with 20 μg of papain (i.n.) for two times, once a day. One day last challenge, epithelial cells were sorted and cultured with 200 ng/ml papain. After 2 h, IL-28B (100 ng/ml) was added and cocultured for 24 h. (D) The concentration of IL-25 and TSLP in supernatant were determined by ELISA. (E) The phosphorylation of Erk (~ 42.4 kDa) and total Erk (~ 42.4 kDa) in epithelial cells were analyzed by Western Blot. Data are representative of three or more independent experiments. Data are the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. i.n., intranasally injection.
