Figure 3 | Scientific Reports

Figure 3

From: Investigating the optical clearing effects of 50% glycerol in ex vivo human skin by harmonic generation microscopy

Figure 3

Ex vivo HGM images of Case E100-I90-2, with 100% glycerol immersion. (a) En face HGM images of (1) Top layer of the SC, (2) Bottom layer of the SC, (3) Middle layer of the viable epidermis, (4) Basal layer, (5) Middle layer of the papillary dermis, and (6) Top layer of the reticular dermis. Scale bar = 100 μm. (b) Transverse HGM images. In the layer of SC, the strong two-photon fluorescence leakage of the residue marking dye into the SHG channel is observable and is not included in our SHG analysis. The significant depth difference of the corresponding layer is attributed to the sample shrinkage post glycerol immersion. Arrow 1: Epidermis; Arrow 2: Dermis.

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