Table 4 Variation of skin structure, THG intensity, and SHG intensity after 100% glycerol topical application for 30 min.

From: Investigating the optical clearing effects of 50% glycerol in ex vivo human skin by harmonic generation microscopy

Case E100-T30

Depth or thickness (μm)

Thickness of SC

Thickness of viable epidermis

Depth of basal layer

Depth of middle papillary dermis layer

Depth of Top reticular dermis layer

Before (N = 10)

13.5 \(\pm \) 0.9

17.3 \(\pm \) 4.3

30.8 \(\pm \) 4.7

60.1 \(\pm \) 6.0

88.7 \(\pm \) 13.2

Post-glycerol (N = 10)

13.5 \(\pm \) 2.6

21.6 \(\pm \) 2.4*

35.1 \(\pm \) 3.3*

67.0 \(\pm \) 8.0*

98.1 \(\pm \) 16.9

THG intensity

Bottom of SC

Middle viable epidermis layer

Basal layer

Middle papillary dermis layer

Top reticular dermis layer

After the applying glycerol

(***)

(***)

(***)

(***)

SHG intensity

Middle papillary dermis layer

Top reticular dermis layer

SHG maximum

  

After the applying glycerol

(*)

  
  1. −: no significant difference, : Increased significantly, : Decreased significantly, *P < 0.05, ** P < 0.01, *** P < 0.001.