Figure 4 | Scientific Reports

Figure 4

From: A combined NMR and EPR investigation on the effect of the disordered RGG regions in the structure and the activity of the RRM domain of FUS

Figure 4

Left panels—1H-15 N HSQC NMR spectra of MTSL-labelled (red) and unlabelled (black) FUS165–422 mutants (A) D180R1, (B) D258R1, (C) A349R1, (D) S360R1 and (E) S412R1 with the assignment of the relevant cross peaks; Central panels—mapping on the protein surface of the RRM domain (PDB ID 2LA6) of the relevant changes in signal intensity. Residues are coloured from red to orange according to PRE magnitude (F–J); unaffected residues are coloured in grey. The labelled position in the RRM is coloured in yellow. Right panels: Paramagnetic relaxation enhancement values reported as normalized intensity ratio (I/I°) for FUS165–422 mutants (F) D180R1, (G) D258R1, (H) A349R1, (I) S360R1 and (J) S412R1.

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