Table 2 Component percentages (%) and correlational times (τC) calculated from simulations (SimLabel software, ref.44) for each spin labeled FUS165–422 mutants in the RRM domain, alone and in presence of hnRNPA2/B1 stem-loop RNA.

From: A combined NMR and EPR investigation on the effect of the disordered RGG regions in the structure and the activity of the RRM domain of FUS

MTSL-lablled mutants

Component 1

Component 2

Component 3

%

τC (ns)

%

τC (ns)

%

τC (ns)

A349R1

15

0.40

50

0.72

35

1.40

A349R1 + RNA

65

0.30

  

35

1.40

S360R1

72

0.48

  

28

2.17

S360R1 + RNA

27

0.57

35

0.78

38

2.13

  1. Standard deviations on components percentages and τC were estimated equal to ± 4% and ± 0.09 ns respectively.
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