Figure 4
Frequency of granzyme B+ and IFNγ+ cells in PD-1- and TIM-3-positive and negative CD8+ T cell subsets in healthy donors and multiple myeloma patients. Graphs A-B show relative counts of granzyme B+ cells in (A) PD-1- and (B) TIM-3-positive (white boxes) and negative (grey boxes) CD8+ T cells in PB of healthy donors (HD PB, n = 15) and in PB and BM of MM patients (MM PB and MM BM, respectively, n = 26). Graphs C–F show relative counts of IFNγ+ cells in (C,E) PD-1- and (D, F) TIM-3-positive (white boxes) and negative (grey boxes) CD4+ (C,D) and CD8+ (E,F) T cells in PB of healthy donors (HD PB, n = 10) and in PB and BM of MM patients (MM PB and MM BM, respectively, n = 10). Data are expressed as median, interquartile range and range of minimum and maximum values. P values are assessed with Mann–Whitney U-test (*PU < 0.05) between independent groups and sign test (#P < 0.05) between paired groups. Generated using GraphPad Prism 5.0 (GraphPad Software, Inc., San Diego, CA, USA; https://www.graphpad.com/).
