Figure 2 | Scientific Reports

Figure 2

From: Loss of miR-100 and miR-125b results in cancer stem cell properties through IGF2 upregulation in hepatocellular carcinoma

Figure 2

Stemness factors regulates miR-100 and -125b expression. (A) miR-100 and miR-125b expression levels measured after transfection of NANOG, OCT4, and SOX2 with the TaqMan probe. Relative expression was calculated using U6 expression as an internal control. (B) Physical map of the hsa-miR-100 promoter region for the design of chromatin immunoprecipitation (ChIP) primer. The position of the stemness binding motif designed ChIP primer. (C) Stemness factor transcriptional activity was assessed by pGL3-miR-100 promoter luciferase reporter in Hep3B cells. The average (column) ± SD (bar) is indicated (*p < 0.05, **p < 0.01, ***p < 0.001).

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