Figure 4
From: USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy
USP24 removes ubiquitin from Lys391 and Lys400 of the BRD. HA-BRD and its various HA-BRD mutants were expressed in USP24-silenced H1299 cells, following which Western blotting was performed with anti-USP24, anti-HA and anti-actin antibodies (A). The HA-BRD and its mutant, HA-BRD (K391/400R) were expressed in USP24-silenced H1299 cells for IP with anti-HA and anti-myc, and then Western blot with indicated antibodies (B). HA-BRD and the mutant HA-BRD (K391/400R), were expressed inside H1299 cells in the present of cycloheximide, and lysates were collected at the indicated time for Western blotting with anti-HA antibody, and The BRD and mutant BRD were quantitated, and the results were subjected to statistical analysis by t-test, *p < 0.05; **p < 0.01, after three independent experiments (C). The localization of Lys391 and Lys400 in the structure of the BRD are shown (D).
