Figure 1
From: Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism
Primary stromal vascular fraction self-organizes into a 3D adipose tissue spheroid. (a) Time line of differentiation of adipose tissue spheroids after plating in 3D. (b) Representative figures of spheroids after plating in 3D (bar 150 µm). (c) Volume of spheroids was analyzed with and without differentiation cocktail in low and high glucose DMEM over 16 days [n = 48 spheroids (technical replicates) for each condition from 2 individual experiments (biological replicates)]. Differentiated spheroids were stained with (d) DAPI (blue) and ActinGreen or (e) LD540 (bar 50 µm).
