Figure 2
From: Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism
Mass spectrometry lipid analysis of 3D adipose tissue spheroids is a new tool to efficiently study lipid metabolism in vitro. SVF were differentiated and harvested at day 1, day 4, day 6 and day 9. Total lipids were extracted and analysed by MS. (a) Amount of PC normalized to the amount of total lipids. (b) Ratio of NL to polar lipids. (c) Composition of NL. (d) Average number of double bonds. (e) Average chain lengths of NL. (f) Heatmap of TAG species as % of total TAG amount. (g) Heatmap of DAG species as % of total DAG amount. (h) Heatmap of CE species as % of total CE amount (n = 3 individual experiments (biological replicates) each performed in technical triplicates). Data are presented as mean ± SD. Significance was determined using Two Way ANOVA with Dunette correction for multiple comparisons. *p < 0.05, **p < 0.01 and ***p < 0.001.
