Figure 3
From: Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism
Lipid composition of SVF derived spheroids resembles in vivo adipose tissue. Mouse SVF were cultured under 2D or 3D conditions, when fully differentiated lipids were isolated and analysed by MS. Profiles were compared with lipid profiles of freshly isolated mouse adipose tissue. (a) Ratio of neutral lipids to PC and rest and composition of NLs. (b) Average chain length of C-atoms of NLs. (c) Average number of double bonds of NLs. (n = 3 individual experiments (biological replicates) each performed in technical triplicates). Data are presented as mean ± SD. Significance was determined using two way ANOVA with Dunette correction for multiple comparisons. *p < 0.05, **p < 0.01 and ***p < 0.001.
