Figure 4
From: Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism
3D-adipose tissue spheroids can be used to monitor either dietary- or insulin-induced metabolic changes in the lipidome. Differentiated SVF were treated for 14 days with OA, SA or Insulin. Total lipids were extracted and analysed by MS. (a) Experimental timeline. (b) Ratio of NL to polar lipids. (c) Fold change of TAG species. (d) Composition of neutral lipids. (e) Average chain lengths of neutral lipids. (f) Average number of double bonds. (g) TAG and (h) DAG species as percentage of total TAG and DAG (n = 3 individual experiments (biological replicates) each performed in technical triplicates). Data are presented as mean ± SD. Significance was determined using two way ANOVA with Dunette correction for multiple comparisons. *p < 0.05, **p < 0.01 and ***p < 0.001.
