Figure 2 | Scientific Reports

Figure 2

From: Proline-rich tyrosine kinase 2 mediates transforming growth factor-beta-induced hepatic stellate cell activation and liver fibrosis

Figure 2

Inhibition of PYK2, but not FAK, suppresses upregulation of pro-fibrotic CTGF expression on stimuli of TGF-β1. (A, B) qPCR of fibrogenic gene expression in in vitro activated primary mouse hepatic stellate cell (mHSC) (A) and in LX2 (B), treated as indicated and shown as fold change (n = 3). (C) Western blot of α-SMA in mHSC and LX2 after treatments. (D) Western blot of activated forms of both FAK and PYK2, and CTGF in LX2 treated as indicated (n = 3). (E) qPCR of CTGF expression in LX2 that were treated with either of two FAK/PYK2 inhibitors (n = 3). (F, G) After transfection with siRNAs of negative control, FAK or PYK2, qPCR of the indicated gene expression (F) and western blot (G) were performed. Two FAK/PYK2 dual inhibitors were used: PF271, PF-573271; PF228, PF573228. Student's t-test; *p < 0.05, **p < 0.01, and ***p < 0.001.

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