Table 2 Effect of interaction between the post-ovulation stage and the age on the relative mRNA expression of candidate genes for the transcellular and paracellular pathways involved in Ca absorption in the jejunum.

	Genes	ATP2B2*	CLDN12^†	OCLN^†
Age	PO stage
23	0–1	0.16 (± 0.09)^b	0.72 (± 0.07)^b	0.90 (± 0.06)^a
	9–10	0.69 (± 0.09)^a	1.26 (± 0.07)^a	0.62 (± 0.06)^b
	18–19	0.43 (± 0.09)^ab	0.76 (± 0.06)^b	0.71 (± 0.06)^ab
90	0–1	0.18 (± 0.09)^b	0.56 (± 0.07)^b	0.61 (± 0.06)^b
	9–10	0.30 (± 0.13)^ab	0.61 (± 0.10)^b	0.58 (± 0.09)^b
	18–19	0.17 (± 0.11)^b	0.52 (± 0.08)^b	0.76 (± 0.07)^ab
		Source of variation
Age		0.864	0.092	0.002
PO stage		0.040	0.731	0.026
Age × PO stage		0.002	< .0001	0.003

*Genes of the transcellular pathway; ^†genes of the paracellular pathway. PO stage = post-ovulation stage (hour). Relative expression values expressed as Arbitrary Units are presented as LSMeans (± SEM) with n = 10–12 per PO stage; n = 15–19 per age and n = 4–6 per PO stage and age.
^a,bMeans with different superscripts for each effect tested in each model are significantly different (P < 0.05). ATPase plasma membrane Ca²⁺ transporting 2 (ATP2B2); Claudin 12 (CLDN12); Occludin (OCLN).

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